Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4305

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Preparing Acetone Powders

Ed Harlow and David Lane

This protocol was adapted from "Protein Techniques," Appendix II, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

The first 15% of the full text of this article appears below.


MATERIALS

Reagents

caution Acetone

NaCl (0.9%)


METHOD

1. Prepare a fine suspension of tissue or cells in NaCl (0.9%). If a tissue is used, either discard any fibrous material by teasing it away from the remainder of the tissue or . . . [Full Text of this Article]


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