Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4269

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Staining Immunoblots for Total Protein Using Ponceau S

Ed Harlow and David Lane

This protocol was adapted from "Immunoblotting," Chapter 8, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

The first 15% of the full text of this article appears below.


INTRODUCTION

To determine the position of molecular-weight markers and to ensure that efficient transfer of proteins to the blot has occurred, the total composition of the proteins can be determined by staining the membrane with Ponceau S . . . [Full Text of this Article]


MATERIALS

Reagents


METHOD


TROUBLESHOOTING


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