Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4331

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Binding Antibodies to Tissue Sections

Ed Harlow and David Lane

This protocol was adapted from "Staining Tissues," Chapter 6, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

The first 100 words of the full text of this article appear below.


INTRODUCTION

Once tissues are fixed and permeabilized, the antibodies are added. These antibodies can be labeled directly or detected by a labeled secondary reagent. For indirect detection, any reagent that binds specifically to the primary antibody can be "tagged" and used to locate the antibody. The possible reagents include anti-immunoglobulin antibodies, protein A or G, or, if the first antibody is labeled with biotin, streptavidin. They can be labeled with enzymes or gold. The major advantage of indirect detection is that one set of labeled reagents can be used for a number of primary antibodies. Indirect methods normally give stronger signals . . . [Full Text of this Article]


MATERIALS

Reagents


METHOD


TROUBLESHOOTING


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