Protocol

Delivery of dsRNA into Plants by VIGS Methodology

This protocol was adapted from "PTGS Approaches to Large-Scale Functional Genomics in Plants" contributed by Tessa M. Burch-Smith, Jennifer L. Miller, and Savithramma P. Dinesh-Kumar, Chapter 12, in RNAi: A Guide to Gene Silencing (ed. Hannon). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

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INTRODUCTION

Virus-induced gene silencing (VIGS) uses a virus to deliver a sequence from a gene of interest into a host plant. The virus carrying the fragment of the gene of interest must be capable of replication if dsRNA is to be produced. One or two leaves are inoculated with Agrobacterium strains carrying the VIGS vector possessing the gene fragment. The virus then replicates and spreads throughout the plant, mediating silencing. Transient . . . [Full Text of this Article]

MATERIALS

Reagents

Equipment

METHOD