Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4326

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, L.
Right arrow Articles by Cameron, C. E.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Huang, L.
Right arrow Articles by Cameron, C. E.
Related Collections
Right arrow Molecular Biology, general
Right arrow RNA, general
Right arrow RNA Interference (RNAi)/siRNA
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Legend icon

protocolProtocol

Protocol for siRNA-Primed RNA Synthesis

Luyun Huang, John Gledhill, and Craig E. Cameron

This protocol was adapted from "RNA-Dependent RNA Polymerase in Gene Silencing," contributed by Luyun Huang, John Gledhill, and Craig E. Cameron, in RNAi: A Guide to Gene Silencing (ed. Hannon. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

Short interfering RNAs (siRNAs) can be used to prime RNA synthesis by the RNA-dependent RNA polymerase (RdRP). siRNAs can be used by RdRP as primers for specific cellular mRNAs, forming dsRNA products capable of inducing transitive RNAi. For the preparation of siRNAs from Drosophila . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?