Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4324

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Preparation and Fractionation of Drosophila Embryo Extracts for the In Vitro Characterization of RNAi

Luyun Huang, John Gledhill, and Craig E. Cameron

This protocol was adapted from "RNA-Dependent RNA Polymerase in Gene Silencing," contributed by Luyun Huang, John Gledhill, and Craig E. Cameron, in RNAi: A Guide to Gene Silencing (ed. Hannon). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes a method to prepare and fractionate Drosophila embryo extracts. These extracts can be used for the in vitro characterization of RNAi.


MATERIALS

Reagents

caution Bleach (50% v/v)

recipe Hypotonic buffer for Drosophila embryo . . . [Full Text of this Article]

Equipment


METHOD


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