Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4323

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Assay for Primed RNA-Dependent RNA Polymerase Transcription

Luyun Huang, John Gledhill, and Craig E. Cameron

This protocol was adapted from "RNA-Dependent RNA Polymerase in Gene Silencing," contributed by Luyun Huang, John Gledhill, and Craig E. Cameron, in RNAi: A Guide to Gene Silencing (ed. Hannon). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

For short RNA templates with known sequence, products of RNA-dependent RNA polymerase (RdRP)-catalyzed primer extension can be analyzed by denaturing polyacrylamide gel electrophoresis.


MATERIALS

Reagents

caution[{alpha}-32P]ATP (see radioactive material)

Bovine serum albumin (BSA), acetylated

caution Dithiothreitol (DTT) . . . [Full Text of this Article]

Equipment


METHOD


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