Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4322

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An Assay for RNA-Dependent RNA Polymerase Activity

Luyun Huang, John Gledhill, and Craig E. Cameron

This protocol was adapted from "RNA-Dependent RNA Polymerase in Gene Silencing," contributed by Luyun Huang, John Gledhill, and Craig E. Cameron, in RNAi: A Guide to Gene Silencing (ed. Hannon). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2003, USA

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol provides a quick way to assay for RNA-dependent RNA polymerase (RdRP) activity in crude protein samples. RdRP transcription products remain at the origin during paper chromatography. Therefore, RdRP activity is monitored by providing RdRP with a . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


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