Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4320

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Detection of si/miRNA in C. elegans by Northern Blot

René F. Ketting, Marcel Tijsterman, and Ronald H.A. Plasterk

This protocol was adapted from "RNAi in C. elegans," contributed by René F. Ketting, Marcel Tijsterman, and Ronald H.A. Plasterk, Chapter 4, in RNAi: A Guide to Gene Silencing (ed. Hannon). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol uses northern blot technology to detect short interfering RNAs (siRNAs) or micro-RNAs (miRNAs) in RNA preparations from Caenorhabditis elegans (see Isolation of RNA from C. elegans). siRNAs can also be detected by RNase protection (see Detection of si/miRNA in C. elegans Using RNase . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


TROUBLESHOOTING


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Detection of siRNA in C. elegans Using RNase Protection
René F. Ketting, Marcel Tijsterman, and Ronald H.A. Plasterk
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