Protocol

Introduction of Double-Stranded RNA in C. elegans by Soaking

This protocol was adapted from "RNAi in C. elegans," contributed by René F. Ketting, Marcel Tijsterman, and Ronald H.A. Plasterk, Chapter 4, in RNAi: A Guide to Gene Silencing (ed. Hannon). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.

INTRODUCTION

Double-stranded RNA (dsRNA) can be introduced into Caenorhabditis elegans by soaking the animals in a solution of dsRNA. Alternative methods are dsRNA injection (see Introduction of Double-stranded RNA in C. elegans by Injection) and feeding the animals with bacteria that produce dsRNA (see Introduction of Double-Stranded RNA in C. elegans by Feeding. . . [Full Text of this Article]

MATERIALS

Reagents

Equipment

METHOD

TROUBLESHOOTING