Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4180

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Ethyl Methane Sulfonate (EMS) Mutagenesis

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "EMS Mutagenesis," Techniques and Protocols 21, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes mutagenesis of yeast with ethyl methane sulfonate (EMS). It causes approximately 40-70% cell death in most haploid laboratory strains, a level of cell killing that is commonly used in mutant hunts with haploid strains.

Extreme caution!

EMS is a powerful carcinogen. When performing experiments using EMS, always use a fume hood, and decontaminate the work area by wiping down surfaces and equipment with 1 N NaOH and rinsing with water. Decontaminate EMS-containing liquids by adding NaOH to 1 N . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


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