Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4178

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Preparation of Yeast Cells for Flow Cytometry

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "Flow Cytometry of Yeast DNA," Techniques and Protocols 19, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.


INTRODUCTION

Flow cytometry is used to analyze the quantity of DNA in cells. Since the DNA content of cells varies through the cell cycle, this information can provide an indication of cell cycle progression. This protocol uses SYTOX Green staining, as described by Haase and Reed (2002).


MATERIALS

Reagents

Chosen yeast culture

Ethanol

recipe caution Pepsin solution, 5 mg/ml

recipe caution caution RNase solution in . . . [Full Text of this Article]

Equipment


METHOD


TROUBLESHOOTING


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