Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4168

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Actin Staining in Fixed Yeast Cells

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "Actin Staining in Fixed Cells," Techniques and Protocols 13, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.


INTRODUCTION

Phalloidin binds specifically to F-actin, and fluorescent-tagged phalloidin stains the actin skeleton in cells in a manner that is very close to the staining pattern seen using anti-actin antibody.


MATERIALS

Reagents

Appropriate yeast culture

caution Formaldehyde (Polysciences, EM-grade), 10%

Appropriate liquid growth medium

recipe caution Mounting solution

recipe PBS

caution . . . [Full Text of this Article]

Equipment


METHOD


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