Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4165

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Yeast Vital Stains: Visualizing Vacuoles and Endocytic Compartments with FM4-64

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "Yeast Vital Stains," Techniques and Protocols 11, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.


INTRODUCTION

Various vital stains are available for yeast. This protocol describes visualization of vacuoles and endocytic compartments with FM4-64, a lipophilic styryl dye that is taken up by yeast cells . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


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