Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4162

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Random Spore Analysis in Yeast

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "Random Spore Analysis," Techniques and Protocols 10, in Methods in Yeast Genetics, 2005 edition by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.


INTRODUCTION

Random spore analysis is used to examine meiotic recombination without performing tetrad analysis.


MATERIALS

Reagents

Appropriate yeast culture

cautionß-glucuronidase, 100 units/µl (Sigma)

recipe Sporulation medium

recipe Synthetic Complete (SC)-arg plates, containing 60 µg/ml of canavanine

recipe YPD plate

Equipment

Conical polystyrene tube, 15 ml

Dowel, sterile

Glass beads, . . . [Full Text of this Article]


METHOD


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