Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4161

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Plate Assay for Carboxypeptidase Y in Yeast Cells

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "Plate Assay for Carboxypeptidase Y," Techniques and Protocols 9, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.


INTRODUCTION

Carboxypeptidase Y is a vacuolar enzyme whose activity can be used to screen yeast colonies. It cleaves the ester linkage in N-acetyl-DL-phenylalanine . . . [Full Text of this Article]


MATERIALS

Reagents


METHOD


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