Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4160

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Assay of ß-Galactosidase in Yeast: Freeze/Thaw Assay by Chemiluminescence in 96-Well Plates

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "Assay of ß-Galactosidase in Yeast," Techniques and Protocols 8, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.


INTRODUCTION

There are several methods for the in vitro assay of ß-galactosidase in yeast. In the method described here, the cells are lysed by a simple freeze/thaw protocol and treated with a chemiluminescent substrate. . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


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Related Protocol

Assay of ß-Galactosidase in Yeast: Freeze/Thaw Assay by Chemiluminescence in Microcentrifuge Tubes
David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern
CSH Protocols 2006: 4159. [Extract] [Full Text]