Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4158

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Assay of ß-Galactosidase in Yeast: Permeabilized Cell Assay

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "Assay of ß-Galactosidase in Yeast," Techniques and Protocols 8, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.


INTRODUCTION

There are several methods for the in vitro assay of ß-galactosidase in yeast. In the method described here (Guarente 1983), the cells are permeabilized to allow the substrate to enter the cells and the activity is normalized to the number of cells assayed. This method is used to assess changing levels . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


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