Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4155

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Amberg, D. C.
Right arrow Articles by Strathern, J. N.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Amberg, D. C.
Right arrow Articles by Strathern, J. N.
Related Collections
Right arrow Yeast
Right arrow Molecular Biology, general
Right arrow CSHL Yeast Genetics and Genomics Course
Right arrow RNA
Right arrow RNA, general
Right arrow RNA Purification
Right arrow Genetics, general
Right arrow Yeast Genetics
Right arrow Laboratory Organisms, general
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Legend icon

protocolProtocol

Yeast RNA Isolation: Small-Scale

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "Yeast RNA Isolations," Techniques and Protocols 6, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes rapid, small-scale yeast RNA isolation. It is based on the work of Schmitt et al. (1990). Note that all containers should be washed in RNase Away (Invitrogen) or dry baked for 24 hours at 160°C. All aqueous reagents should be made with 1:1000 volume of DEPC before autoclaving, to inactivate RNases.


MATERIALS

Reagents

recipe AE buffer, chilled

Appropriate yeast culture

caution Dry ice/95% ethanol bath

Ethanol 80% and 100%

caution Phenol equilibrated in AE buffer (pH 5.2)

caution Phenol (pH 5.2):chloroform:isoamyl alcohol (25:24:1)

recipe 10% SDS

recipe Sodium acetate (3 M, pH 5.2)

recipe YPD medium

Equipment

Shaking incubator, preset to an . . . [Full Text of this Article]


METHOD


TROUBLESHOOTING


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?