Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4152

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Yeast Protein Extracts

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "Yeast Protein Extracts," Techniques and Protocols 4, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol is a simple, reliable method for the preparation of yeast protein extracts for analysis by polyacrylamide gel electrophoresis (PAGE) and Western blotting. It works for both growing and stationary . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


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