Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4517

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Delivery of dsRNA into Drosophila Embryos by Gene Gun

Richard W. Carthew

This protocol was adapted from "RNAi in Drosophila" contributed by Richard W. Carthew, Chapter 17, in RNAi, A Guide to Gene Silencing (ed. Hannon). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 100 words of the full text of this article appear below.


INTRODUCTION

In this method, subcellular-sized particles are accelerated to high velocity to carry double-stranded RNA (dsRNA) into Drosophila embryos. The major advantage of this procedure over microinjection (Microinjection of dsRNA into Drosophila Embryos) is that particle bombardment is easier and faster to perform. In addition, the mechanical trauma received is far less than by microinjection, allowing better survival of embryos and fewer phenotypic artifacts. The principle of particle bombardment is to employ a high-velocity stream of helium gas to accelerate gold particles coated with dsRNA to sufficient velocities to penetrate embryos. This protocol describes the use of the Helios . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


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Synthesis of dsRNA for RNAi in Drosophila: PCR Template Method
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CSH Protocols 2006: 4514. [Extract] [Full Text]

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