Protocol

Mass Culture of Freshwater Hypotrichs

This protocol was adapted from "Culture and Manipulation of Hypotrichous Ciliated Protozoa," Chapter 17, in Cells (eds. Spector et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1998. This three-volume set is now out of print; however, some of the microscopy methods were republished in Basic Methods in Microscopy, by David L. Spector and Robert D. Goldman.

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INTRODUCTION

This protocol describes the culture of freshwater hypotrichs using the alga Chlorogonium elongatum as a food source. Most freshwater hypotrichs (including Oxytricha nova, O. fallax, and O. trifallax; Euplotes aediculatus and E. eurystomous; and Stylonychia lemnae) can be grown to high . . . [Full Text of this Article]

MATERIALS

Reagents

Equipment

METHOD