Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot3804

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Chemical Sequencing

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 100 words of the full text of this article appear below.


INTRODUCTION

This protocol is based on the classic base-specific cleavage method of DNA sequencing devised in 1977 by Maxam and Gilbert.


MATERIALS

When the quantity of radiolabeled DNA is limiting, it is possible to determine an unambiguous DNA sequence by carrying out only four (C, C+T, A+G, and G) of the five reactions detailed in the flowchart on page 12.65 of the print version of the manual.

When the quantity of radiolabeled DNA is limiting, it is possible to determine an unambiguous DNA sequence by carrying out only four (C, C+T, A+G, and G) of the five reactions detailed in the . . . [Full Text of this Article]


METHOD


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