Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot3794

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Loading and Running DNA Sequencing Gels

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 100 words of the full text of this article appear below.


INTRODUCTION

The sets of nested DNA fragments generated by DNA sequencing methods are resolved by electrophoresis through thin denaturing polyacrylamide gels. WARNING Large voltages are passed through DNA-sequencing gels at substantial amperages. More than enough current is used in these gels to cause severe burns, ventricular fibrillation, central respiratory arrest, and asphyxia due to paralysis of the respiratory muscles. Make sure that the gel boxes used for electrophoresis are well insulated, that all buffer chambers are covered, and that the box is used on a stable bench top that is dry. Always turn off the power to the box before sample . . . [Full Text of this Article]


MATERIALS


METHOD

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