Protocol

Oviduct Transfer

This protocol was adapted from "Surgical Procedures," Chapter 6, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 100 words of the full text of this article appear below.

INTRODUCTION

This protocol describes oviduct transfer of mouse embryos. It is based on the Whittingham method, which applied a well described procedure for the rat to the mouse. It is best to practice this procedure first on a cadaver and then on an anesthetized 0.5-dpc pseudopregnant mouse using a dye solution or blue Affigel beads rather than embryos to gain experience in finding the opening of the oviduct (infundibulum). The position of the infundibulum is relatively invariant from mouse to mouse.

MATERIALS

Reagents

Appropriate anesthetic

Beads, blue Affigel (BioRad)

Epinephrine (Sigma) (optional)

Ethanol, 70%

M2 medium or other HEPES-buffered medium at room temperature

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Equipment

METHOD