Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4288

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Labeling Monoclonal Antibodies by Biosynthesis

Ed Harlow and David Lane

This protocol was adapted from "Handling Antibodies," Chapter 4, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

The first 15% of the full text of this article appears below.


INTRODUCTION

This method for tagging monoclonal antibodies involves growing hybridomas in the presence of radioactive amino acids. This protocol can be particularly useful when conventional labeling techniques cause the antibody to lose activity. The labeled antibodies that result are essentially identical to the unlabeled antibodies.


MATERIALS

Reagents

Hybridoma cells that produce the monoclonal antibody of . . . [Full Text of this Article]

Equipment


METHOD


TROUBLESHOOTING


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