Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4395

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Making Injection Pipettes

Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer

This protocol was adapted from "Production of Transgenic Mice," Chapter 7 of Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes the determination of useful settings with the Sutter Puller P-97 to make injection pipettes for microinjection.


MATERIALS

Equipment

Forceps

Glass capillary tubing with an internal glass filament (e.g., W-P Instruments TW100F; Clark Electromedical Instruments GC 100TF-15)

Alternatively, commercially available . . . [Full Text of this Article]


METHOD


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