Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4456

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Standard Ethanol Precipitation of DNA in Microcentrifuge Tubes

Joseph Sambrook and David W. Russell

This protocol was adapted from "Commonly Used Techniques in Molecular Cloning," Appendix 8, in Molecular Cloning, Volume 3, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001.

The first 100 words of the full text of this article appear below.


INTRODUCTION

This protocol describes the standard method to recover nucleic acids from aqueous solutions by precipitation of DNA with ethanol. Subnanogram amounts of DNA (and RNA) can be quantitatively precipitated with ethanol, collected by centrifugation, and redissolved within minutes.


MATERIALS

Reagents

DNA solution to be precipitated

Ethanol, 100% (ice-cold) and 70%

caution Isopropanol (optional; see Step 9)

Sodium acetate (stock concentration 3 M, pH 5.2)

Alternatively, stock concentrations of ammonium acetate (10 M), sodium chloride (5 M), or lithium chloride (8 M) may need to be used (see Step 2).

MgCl2 (optional; see Step 3)

recipe TE (pH 8.0 in Step 1; pH 7.6-8.0 in . . . [Full Text of this Article]

Equipment


METHOD


TROUBLESHOOTING


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