Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4454

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Estimation of Cell Number by Hemocytometry Counting

Joseph Sambrook and David W. Russell

This protocol was adapted from "Commonly Used Techniques in Molecular Cloning," Appendix 8, in Molecular Cloning, Volume 3, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes a method for estimation of mammalian cell number in a defined volume of medium using a hemocytometer. Automated methods using cell-counting devices such as those produced by Coulter are desirable when large numbers of individual samples are to be counted.


MATERIALS

Reagents

Appropriate growth medium

Cell culture to be counted

Ethanol, 70%

Equipment

Hemocytometer

Lens paper

Microscope

Pasteur pipettes


METHOD

  1. Trypsinize the cells (please see Step 18 of Tetracycline as Regulator of Inducible Gene Expression: Stage 1: Stable Transfection of Fibroblasts with pTet-tTAk) and resuspend them . . . [Full Text of this Article]

TROUBLESHOOTING


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Related Protocol

Tetracycline as Regulator of Inducible Gene Expression • Stage 1: Stable Transfection of Fibroblasts with pTet-tTAk
Joseph Sambrook and David W. Russell
CSH Protocols 2006: 3986. [Extract] [Full Text]



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Inoculation and Passaging of Mammalian Monolayer Cell Cultures
CSH Protocols, August 1, 2008; 2008(9): pdb.prot4347 - pdb.prot4347.
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