Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4433

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TUNEL Staining of Adherent Cells to Detect Apoptosis

Shailaja Kasibhatla, Gustavo P. Amarante-Mendes, Deborah Finucane, Thomas Brunner, Ella Bossy-Wetzel, and Douglas R. Green

This protocol was adapted from "Apoptosis Assays," Chapter 15, in Cells (eds. Spector et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1998. This three-volume set is now out of print; however, some of the microscopy methods were republished in Basic Methods in Microscopy, by David L. Spector and Robert D. Goldman.

The first 15% of the full text of this article appears below.


INTRODUCTION

The TUNEL (terminal deoxynucleotide transferase (TdT)-mediated dUTP- digoxigenin nick-end labeling) method is based on the in situ labeling of DNA fragmentation sites in nuclei of intact fixed cells. This protocol describes the application of this technique to adherent cells.


MATERIALS

Reagents

Apoptosis inducer(s) to be tested

Cell . . . [Full Text of this Article]

Equipment


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