Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4371

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nagy, A.
Right arrow Articles by Behringer, R.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Nagy, A.
Right arrow Articles by Behringer, R.
Related Collections
Right arrow CSHL Molecular Embryology of the Mouse Course
Right arrow Cell Biology, general
Right arrow Cell Culture
Right arrow Laboratory Organisms, general
Right arrow Mouse
Right arrow Developmental Biology
Right arrowRelated Protocols
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Legend icon

protocolProtocol

Roller Culture of Postimplantation Embryos

Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer

This protocol was adapted from "Isolation, Culture, and Manipulation of Postimplantation Embryos," Chapter 5 (Protocol 7, procedure provided by Patrick Tam), in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 100 words of the full text of this article appear below.


INTRODUCTION

This protocol describes roller culture of postimplantation embryos. By this method, embryos from 6.5 to 11.5 days post coitum (dpc) can be isolated, manipulated and cultured outside the uterus. Normal development in vitro will occur for 48 hours when embryos up to early somite stages are used, and for 24 to 36 hours when older embryos are used.


MATERIALS

Reagents

Dulbecco’s modified Eagle’s medium (DMEM)

Gas mixture (see table in Step 5)

Mouse embryos (6.5-11.5 dpc) (see Isolating Postimplantation Embryos: Early Primitive-Streak-Stage, Isolating Postimplantation Embryos: Late Primitive-Streak-Stage, Isolating Postimplantation Embryos: Early Neural-Fold-Stage, Isolating Postimplantation Embryos: Early Somite-Stage, depending . . . [Full Text of this Article]

Equipment


METHOD


Troubleshooting


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Protocols

Isolating Postimplantation Embryos: Early Primitive-Streak-Stage
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols 2006: 4363. [Extract] [Full Text]

Isolating Postimplantation Embryos: Late Primitive-Streak-Stage
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols 2006: 4364. [Extract] [Full Text]

Isolating Postimplantation Embryos: Early Neural-Fold-Stage
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols 2006: 4365. [Extract] [Full Text]

Isolating Postimplantation Embryos: Early Somite-Stage
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols 2006: 4366. [Extract] [Full Text]

Preparation of Rat Serum
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols 2006: 4372. [Extract] [Full Text]