Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4364

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Isolating Postimplantation Embryos: Late Primitive-Streak-Stage

Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer

This protocol was adapted from "Isolation, Culture, and Manipulation of Postimplantation Embryos," Chapter 5, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

Isolation of postimplantation-stage embryos allows one to study normal development as well as genetic mutations which cause postimplantation defects. This protocol describes a method for isolating late primitive-streak-stage embryos (~7.5 days post coitum [dpc]).


MATERIALS

Reagents

Dulbecco’s modified Eagle’s medium (DMEM)

Fetal bovine . . . [Full Text of this Article]

Equipment


METHOD


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