Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4352

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Intraperitoneal (IP) Injection

Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer

This protocol was adapted from "Production of Transgenic and Chimeric Mice: General Issues," Chapter 3, in Manipulating the Mouse Embryo, 3rd edition, by Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol describes intraperitoneal (IP) injection, which is the typical means of introducing most compounds, such as hormones and anesthetics, into the mouse.


MATERIALS

Reagents

Mouse

Equipment

Hypodermic needle, 26- or 30-gauge, 1/2 inch (smaller-size . . . [Full Text of this Article]


METHOD


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