Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4337

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Detection of Gold-Labeled Reagents

Ed Harlow and David Lane

This protocol was adapted from "Staining Tissues," Chapter 6, in Using Antibodies by Ed Harlow and David Lane. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1999.

The first 100 words of the full text of this article appear below.


INTRODUCTION

Gold labels give higher resolution than enzyme-based methods and avoid the problems of substrate preparation and endogenous enzyme activity. Until recently, the gold labels lacked sensitivity at the level of light microscopy, but the recent development of the photochemical silver method of amplification has overcome this problem. Unamplified gold labels can be detected under the light microscope using bright-field illumination where the label ranges from pale pink to deep red, depending on the strength of the reaction. Nomarski differential interference contrast microscopy makes the label appear dark red to black. With the silver enhancement method, the gold particles become coated . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


TROUBLESHOOTING


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Ultrastructural Immunochemistry
CSH Protocols, June 1, 2008; 2008(7): pdb.top47 - pdb.top47.
[Abstract] [Full Text]