Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4319

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Detection of siRNA in C. elegans Using RNase Protection

René F. Ketting, Marcel Tijsterman, and Ronald H.A. Plasterk

This protocol was adapted from "RNAi in C. elegans," contributed by René F. Ketting, Marcel Tijsterman, and Ronald H.A. Plasterk, Chapter 4, in RNAi: A Guide to Gene Silencing (ed. Hannon). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol uses RNase protection to detect short interfering RNAs (siRNAs) in RNA preparations from Caenorhabditis elegans (see Isolation of RNA from C. elegans). siRNAs can also be detected by northern blot (see Detection of si/miRNA in C. elegans by Northern Blot). However, the RNase protection assay seems to be more sensitive.


MATERIALS

Reagents

caution. . . [Full Text of this Article]


METHOD


TROUBLESHOOTING


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Detection of si/miRNA in C. elegans by Northern Blot
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