Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4196

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Separating Proteins Using Ion-Exchange Chromatography

This protocol was adapted from "Ion-Exchange Chromatography," contributed by Bengt Westerlund, Chapter 6, in Purifying Proteins for Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2004.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol details the practical considerations of an ion-exchange chromatography (IEC) experiment. The choice of what type of ion exchanger to use, as well as the composition of the buffers used in this experiment, should be determined prior to beginning this protocol. These decisions are informed by the results of related IEC protocols that are cross-referenced herein.


MATERIALS

Reagents

Binding buffer

Elution buffer

The composition of the binding and elution buffers should be based on the results of any one of the following protocols: Selection of an Ion Exchanger: Determining the pI of a Protein . . . [Full Text of this Article]

Equipment


METHOD


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