Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4181

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Tetrad Dissection

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "Tetrad Dissection," Techniques and Protocols 22, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 100 words of the full text of this article appear below.


INTRODUCTION

This protocol describes dissection of yeast tetrads. Tetrad analysis is useful for linkage studies and for constructing strains for genetic and biochemical experiments.


MATERIALS

Reagents

Appropriate yeast cultures

Plates or liquid medium for sporulation

recipe YPD medium plates

caution Zymolyase solution (zymolase 100T, ICN), 0.05% in 1 M sorbitol, freshly prepared

Equipment

Incubator at 30°C

Micromanipulator

Microscope

Sterile toothpicks (to be used for cultures sporulated on plates)


METHOD

1. Sporulate yeast cells on either appropriate plates or liquid medium. Examine the sporulated cultures to confirm that tetrads have been produced.

Cultures showing less than 5% tetrads are difficult to dissect.

2. Prepare a fresh solution of . . . [Full Text of this Article]


TROUBLESHOOTING


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