Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4171

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Inducing Yeast Cell Synchrony: {alpha}-Factor Arrest Using Low Cell Concentration

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "Cell Synchrony," Techniques and Protocols 17, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.


INTRODUCTION

It is sometimes desirable to have an entire population of yeast cells that is growing synchronously or is arrested at a unique point in the cell cycle. MATa cells will arrest at START in the cell cycle in response to the mating . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


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