Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot4150

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Isolation of Plasmid DNA from Yeast Cells: A Ten-Minute Preparation

David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern

This protocol was adapted from "Yeast DNA Isolations," Techniques and Protocols 3, in Methods in Yeast Genetics, 2005 edition, by David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 15% of the full text of this article appears below.


INTRODUCTION

This protocol (modified from Hoffman and Winston 1987) describes the purification release of plasmid DNA from yeast cells. The resulting plasmid DNA can be used to transform Escherichia coli or yeast. The procedure can be adapted for the isolation . . . [Full Text of this Article]


MATERIALS

Reagents

Equipment


METHOD


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