Cite as: Cold Spring Harb. Protoc.; 2008; doi:10.1101/pdb.prot4966

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Phenotypic Analysis of Arabidopsis Mutants: Ethylene Hormone Response

Paul B. Larsen and Caren Chang

This protocol was adapted from "How to Analyze a Mutant Phenotypically," Chapter 4, in Arabidopsis: A Laboratory Manual (eds. Weigel and Glazebrook). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2002.

The first 100 words of the full text of this article appear below.


INTRODUCTION

Ethylene is one of the five classic plant hormones, having profound effects at all stages of plant growth and development from seed germination to tissue senescence. Tremendous strides have been made in our understanding of the molecular components of the ethylene signal transduction pathway through the identification of mutants having altered responses to ethylene. These mutants were isolated by screening for seedlings with an altered triple-response phenotype, which is manifested upon exposure to high levels of ethylene in the dark. The triple response consists of the formation of a pronounced apical hook, along with a shortening and thickening of both . . . [Full Text of this Article]


RELATED INFORMATION


MATERIALS

Reagents

Equipment


METHOD

Seed preparation

Ethylene Treatment

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