Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.ip26

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A Sealed Preparation for Long-Term Observations of Cultured Cells: Details of Support Slide Construction

Greenfield Sluder, Joshua J. Nordberg, Frederick J. Miller, and Edward H. Hinchcliffe

Adapted from "A Sealed Preparation for Long-Term Observations of Cultured Cells," Chapter 18, in Live Cell Imaging (eds. Goldman and Spector). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

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The aluminum support slide can be fabricated by a professional machine shop. The dimensions of the slide are shown in Figure 1 ; it consists of a 1 x 3-inch aluminum slide 3 mm thick, with a square hole cut in its center . . . [Full Text of this Article]

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