Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.ip5

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The Use of Mass Spectrometry in Proteomics: Database Searching

David M. Schieltz, Michael P. Washburn, and Lara G. Hays

The first 15% of the full text of this article appears below.

Searches are not constrained for only tryptic peptides, and indexed databases (databases only containing tryptic peptides) are not used. In cases where there are very complex mixtures, such as cell lysates, nonspecific cleavages can occur. Therefore, nontryptic peptides would be missed in the database search. If tryptic peptides . . . [Full Text of this Article]


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