Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4026

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Recovery of DNA from Low-melting-temperature Agarose Gels: Enzymatic Digestion with Agarase

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

A fragment of gel containing a band of DNA is excised and digested with agarase, which hydrolyzes the polymer to disaccharide subunits. The released DNA is then purified by phenol extraction and ethanol precipitation. The method works well for DNAs ranging in size from <5 kb to >20 kb.


MATERIALS

Agarase

DNA sample

recipe DNA staining solution

For a discussion of staining agarose gels, please see Detection of . . . [Full Text of this Article]


METHOD


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