Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4025

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Recovery of DNA from Low-melting-temperature Agarose Gels: Organic Extraction

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

DNA fragments separated by electrophoresis through gels cast with low-melting-temperature agarose are recovered by melting the agarose and extracting the resulting solution with phenol:chloroform. The protocol works best for DNA fragments ranging in size from 0.5 kb to 5 kb.


MATERIALS

recipe 1x TAE electrophoresis buffer

recipe 6x Gel-loading buffer

recipe caution Ammonium acetate (10 M)

Optional, please see Step 3.

caution Chloroform

DNA sample

recipe DNA staining solution

For a . . . [Full Text of this Article]


METHOD


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