Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4023

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Recovery of DNA from Agarose and Polyacrylamide Gels: Electroelution into Dialysis Bags

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 100 words of the full text of this article appear below.


INTRODUCTION

A messy but reliable technique that works well for DNAs ranging in size from 200 bp to >50 kb.


MATERIALS

recipe 0.25x TBE electrophoresis buffer

Other electrophoresis buffers such as TAE or 0.5x TBE can be used for electroelution of DNA fragments from agarose and polyacrylamide gels. Buffers are used at reduced strength (0.25-0.5x) to increase the rate at which the DNA migrates through the gel.

recipe 0.25x TBE electrophoresis buffer containing 0.5 µg/ml ethidium bromide

DNA sample

recipe DNA staining solution

Ethanol

Optional, please see Step 5.

caution Phenol:chloroform (1:1, v/v)

Restriction endonucleases

Please see Step 4.

recipe caution Sodium acetate (3 M, pH 5.2)


METHOD

1. . . . [Full Text of this Article]


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