Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot3996

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Analysis of Recombinant Bacteriophage M13 Clones

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

A rapid method to analyze the size of the single-stranded DNA of M13 recombinants.


MATERIALS

recipe 20x SSC

E. coli F' strain

Bacteriophage M13 nonrecombinant vector, grown as well-isolated plaques in top agarose

Bacteriophage M13 recombinant plaques in top agarose

Prepared as described in Cloning into Bacteriophage M13 Vectors.

recipe Gel-loading buffer IV

recipe caution SDS (2% w/v)

Single-stranded recombinant bacteriophage M13 DNA

Choose . . . [Full Text of this Article]


METHOD


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