Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot3994

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Growing Bacteriophage M13 in Liquid Culture

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

Most manipulations with M13, including preparations of viral stocks and isolation of single- and double-stranded DNAs, begin with small-scale liquid cultures that are infected with an M13 plaque, picked from an agar plate.


MATERIALS

recipe 2x YT medium containing 5 mM MgCl2

E. coli F' strain, grown as well-isolated colonies on an agar plate

Bacteriophage M13 plaques plated onto an agar or agarose plate

Please see either Plating Bacteriophage M13or Cloning into Bacteriophage M13 Vectors.

recipe LB containing tetracycline or kanamycin

These media are needed only if a . . . [Full Text of this Article]


METHOD


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