Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot3826

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Removal of Oligonucleotides and Excess dNTPs from Amplified DNA by Ultrafiltration

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

In this protocol, ultrafiltration through Centricon or Microcon concentrators is used to remove unused primers, primer-dimers, and NTPs from preparations of amplified DNA.


MATERIALS

Amplification reaction products (20-200 µl)

caution Chloroform

Optional, please see Step 7.

Ethanol

Optional, please see Step 5.

recipe caution Sodium acetate (3 M)

recipe TE (pH 8.0)


METHOD

1. Place 2 ml . . . [Full Text of this Article]


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