Cite as: Cold Spring Harb. Protoc.; 2006; doi:10.1101/pdb.prot3772

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Removal of Cross-reactive Antibodies from Antiserum: Pseudoscreening

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

Antibodies directed against bacterial and phage-encoded proteins can be removed from sera by incubation with nitrocellulose filters impregnated with lysates of uninfected or phage-infected E. coli.


MATERIALS

Antibody preparation that is to be used for screening

This protocol works best when using an IgG fraction, . . . [Full Text of this Article]


METHOD


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