Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot3771

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Preparation of Lysates Containing Fusion Proteins Encoded by Bacteriophage {lambda}: Lytic Infections in Liquid Medium

Joseph Sambrook and David W. Russell

This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. Russell. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2001

The first 15% of the full text of this article appears below.


INTRODUCTION

This rapid method is used to screen bacteriophage {lambda}gt11 clones for the production of immunodetectable fusion proteins. After optimizng the conditions of infection and induction, the method can be used to produce preparative amounts of a fusion protein.


MATERIALS

E. coli strain Y1090hsdR

This strain is available from the ATCC (www.atcc.org).

Bacteriophage {lambda}gt11 recombinant

Prepare stocks of bacteriophage {lambda} recombinants by soaking individual plaques in approx. 1 ml of SM for at least 2 hours at room . . . [Full Text of this Article]


METHOD


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